Antimicrobial activity of Thymus vulgaris Essential oil Against Multidrug-Resistant Klebsiella pneumoniaee Carrying bla NDM gene

Document Type : Original Article

Authors

1 department of microbiology and microbial biotechnology, shahid Beheshti university

2 Microbiology and microbial biotechnology department, Faculty of Life Science and Technology, Shahid Beheshti University

3 Faculty of Science, University of Thi-Qar, Nasiriyah

4 Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University Department of Horticultural Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.

Abstract

According to the World Health Organization, Klebsiella pneumoniae is an opportunistic pathogen that is responsible for a range of infections, including urinary tract infections (UTIs). This particular species is considered a high-priority concern due to the worldwide issue of antimicrobial resistance. Medicinal plants and their essential oils have always had a variety of uses for different purposes, such as treating diseases worldwide. Therefore, they have a high economic value. The essential oil of Thymus vulgaris was assessed as a replacement for antibiotic growth promoters in the diet of quail chicks and in vitro tests. the study aimed to investigate the antibacterial effects of Thymus vulgaris essential oils on Multidrug-resistant Klebsiella pneumoniae clinically isolated, analyze the antibiotic resistance profile and clinical treatment, and the pathogenicity of the NDM-positive gene Klebsiella pneumoniae. A total of 127 clinical specimens believed to be Klebsiella pneumoniae, including sputum, blood, urine, burn, wound, and stool swabs, were obtained from both genders and different age groups. The samples were streaked on different agars, and the bacterial growth was identified by using biochemical tests and the Vitek®2 system to confirm it. The frequency of antibiotic susceptibility was determined using the Vitek®2 system.
Out of 127 clinical samples, K. pneumoniae was recorded in 50 (39%) cases. The highest isolation rate of K. pneumoniae was in urine 17 (34%). Furthermore, females were more affected by 32 (64%) with K. pneumoniae than males 18 (36%), The results showed that Klebsiella pneumoniae isolates were highly resistant to ampicillin (100%), and more sensitive to thymus vulgaris. Molecular detection of The carbapenemase virulence gene, bla NDM was recorded (32%, 16/50). The significant effect of thyme essential oil as an antibacterial agent against K. pneumoniae has made it a promising natural agent for combating microbial infections.

Keywords

References

Ali FA and Ismael RM. (2017). Dissemination of Klebsiella pneumoniae and Klebsiella oxytoca Harboring bla TEM genes isolated from different clinical samples in Erbil City. Diyala Journal of Medicine. 12 (2): 40-51.
Asbaghian S, Shafaghat A, Zarea K, Kasimov F, Salimi F. (2011). Comparison of volatile constituents, and antioxidant and antibacterial activities of the essential oils of Thymus caucasicus, T. kotschyanus and T. vulgaris. Natural Product Communications. 6 (1). Doi: https://doi.org/10.1177/1934578X1100600133.
Aziz PY, Azeez SH, Hama NH, Taha Y. (2022). Antibacterial activity evaluations of Thymus vulgaris essential oil extract against clinically isolated gram-positive and gram-negative pathogens. 14 (2): 164–173.
Aziz SN and Al Marjani MF. (2022). Investigation of bacterial persistence and filaments formation in clinical K. pneumoniaee. ARO-The Scientific Journal of Koya University. 10 (2): 82-86. Doi: https://doi.org/10.14500/aro.10895.
Chang D, Sharma L, Dela Cruz CS, Zhang D. (2021). Clinical epidemiology, risk factors, and control strategies of Klebsiella pneumoniaee infection. Frontiers in Microbiology. 12: 750662. Doi: https://doi.org/10.3389/fmicb.2021.750662.
Chen J, Li J, Huang F, Fang J, Cao Y, Zhang K, Zhou H, Cai J, Cui W, Chen C, Zhang G. (2023). Clinical characteristics, risk factors, and outcomes of Klebsiella pneumoniae developing secondary Klebsiella pneumoniae bloodstream infection. BMC Pulmonary Medicine. 23 (1): 1–11. https://doi.org/10.1186/s12890-023-02394-8
Christaki E, Marcou M, Tofarides A. (2020). Antimicrobial Resistance in Bacteria: Mechanisms, Evolution, and Persistence. Journal of Molecular Evolution. 88 (1): 26-40. Doi: https://doi.org/10.1007/s00239-019-09914-3
Conservation ER, Abbas FM, Jarallah EM. (2023). First identification of NDM-1 Metallo ß – Lactamase among clinical isolates of Klebsiella pneumoniae isolates in Hilla hospitals. Iraq. 11 (2), 130-138.
Dalmolin TV, Bianchini, BV, Rossi GG, Ramos AC, Gales AC, Trindade PA, de Campos MMA.  (2017). Detection and analysis of different interactions between resistance mechanisms and carbapenems in clinical isolates of Klebsiella pneumoniae. Brazilian Journal of Microbiology. 48 (3): 493-498. https://doi.org/10.1016/j.bjm.2017.01.003.
Dehghani N, Afsharmanesh M, Salarmoini M, Ebrahimnejad H. (2019). In vitro and in vivo evaluation of thyme (Thymus vulgaris) essential oil as an alternative for antibiotic in quail diet. Journal of Animal Science. 97 (7): 2901-2913. https://doi.org/10.1093/jas/skz179.
Diniz AF, Santos B, Santos VRL, Mariz WS, Cruz PSC, Silva RL, Paula AFR, Santos JRDA. (2023). Antibacterial activity of Thymus vulgaris (thyme) essential oil against strains of Pseudomonas aeruginosa, Klebsiella pneumoniae, and Staphylococcus saprophyticus isolated from the meat product. Brazilian Journal of Biology. 83: 1-9. Doi: 10.1590/1519-6984.275306.
Dosary SKAl. (2018). Antibacterial effect of Thymus sp . and Boswellia sp. extracts on Streptococcus pneumoniaee and Klebsiella pneumoniae isolates. 17 (5): 133-138. https://doi.org/10.5897/AJB2017.16051
Fani M and Kohanteb J. (2017). In vitro antimicrobial activity of Thymus vulgaris essential oil against major oral pathogens. Journal of Evidence-Based Complementary and Alternative Medicine. 22 (4): 660–666. https://doi.org/10.1177/2156587217700772
Firoozeh F, Mahluji Z, Shams E, Khorshidi A, Zibaei M. (2017). New Delhi metallo-ß-lactamase-1-producing klebsiella pneumoniae isolates in hospitalized patients in Kashan, Iran. Iranian Journal of Microbiology. 9 (5): 283-287. PMCID: PMC5748447.
 

Fournomiti M, Kimbaris A, Mantzourani I, Plessas S, Theodoridou I, Papaemmanouil V, Kapsiotis I, Panopoulou M, Stavropoulou E, Bezirtzoglou EE, Alexopoulos A. (2015). Antimicrobial activity of essential oils of cultivated oregano (Origanum vulgare), sage (Salvia officinalis), and thyme (Thymus vulgaris) against clinical isolates of Escherichia coli, Klebsiella oxytoca, and Klebsiella pneumoniae. Microbial Ecology in Health and Disease. 1: 1-7.Doi: 10.3402/mehd.v26.23289 .

Ghanbarinasab F, Haeili M, Ghanati SN, Moghimi M. (2023). High prevalence of OXA-48-like and NDM carbapenemases among carbapenem resistant Klebsiella pneumoniaee of clinical origin from Iran. Iranian Journal of Microbiology. 15 (5): 609-615. https://doi.org/10.18502/ijm.v15i5.13866.
Han R, Shi Q, Wu S, Yin D, Peng M, Dong D, Zheng Y, Guo Y, Zhang R, Hu F. (2020). Dissemination of carbapenemases (KPC, NDM, OXA-48, IMP, and VIM) among carbapenem-resistant enterobacteriaceae isolated from adult and children patients in China. Frontiers in Cellular and Infection Microbiology. 10: 314. Doi: https://doi.org/10.3389/fcimb.2020.00314.
Horváth G, Horváth A, Reichert G, Böszörményi A, Sipos K, Pandur E. (2021). Three chemotypes of thyme (Thymus vulgaris L.) essential oil and their main compounds affect differently the IL-6 and TNFα cytokine secretions of BV-2 microglia by modulating the NF-κB and C/EBPβ signalling pathways. BMC Complementary Medicine and Therapies. 21 (1): 1-14. Doi: https://doi.org/10.1186/s12906-021-03319-w.
Ibrahim DH, Abdullah BH, Abdulqadir IM. (2022). Detection of multi-drug resistant Klebsiella Pneumoniae from sputum samples among ICU patients utilizing pcr and Vitek2 System. The Journal of University of Duhok. 25 (2): 473-481. Doi: https://doi.org/10.26682/sjuod.2022.25.2.43
Jwair NA, Al-Ouqaili, MTS, Al-Marzooq F. (2023). Inverse association between theeExistence of CRISPR/Cas Systems with antibiotic resistance, extended spectrum β-Lactamase and carbapenemase production in multidrug, extensive drug and pandrug-resistant Klebsiella pneumoniae. Antibiotics. 12 (6). https://doi.org/10.3390/antibiotics12060980.
Kwiatkowski P, Sienkiewicz M, Pruss A, Łopusiewicz Ł, Arszyńska N, Wojciechowska-Koszko I, Kilanowicz A, Kot B, Dołęgowska B. (2022). Antibacterial and anti-biofilm activities of essential Oil compounds against New DelhiMetallo-β-Lactamase-1-producing uropathogenic Klebsiella pneumoniae strains. Antibiotics. 11 (2). Doi: https://doi.org/10.3390/antibiotics11020147
Li Y, Kumar S, Zhang L, Wu H. (2022). Klebsiella pneumoniae and Its antibiotic resistance: A bibliometric analysis. BioMed Research International. Doi: https://doi.org/10.1155/2022/1668789.
Liang Z, Wang Y, Lai Y, Zhang J, Yin L, Yu X, Zhou Y, Li X, Song Y. (2022). Host defense against the infection of Klebsiella pneumoniae: New strategy to kill the bacterium in the era of antibiotics? Frontiers in Cellular and Infection Microbiology. 12: 1050396. Doi: https://doi.org/10.3389/fcimb.2022.1050396
Mohammed AB and Anwar KA. (2022). Phenotypic and genotypic detection of extended spectrum beta lactamase enzyme in Klebsiella pneumoniaee. PLOS ONE. 17 (9): 1–14. Doi: https://doi.org/10.1371/journal.pone.0267221
Muhsin EA, Said A, Al-Jubori SS. (2022). Correlation of type 1 and Type 3 fimbrial genes with the type of specimen and the antibiotic resistance profile of clinically isolated Klebsiella pneumoniae in Baghdad. Al-Mustansiriyah Journal of Science. 33 (3): 1-11. Doi: https://doi.org/10.23851/mjs.v33i3.1129
Nezhadali A, Nabavi M, Rajabian M, Akbarpour M, Pourali P, Amini F. (2014). Chemical variation of leaf essential oil at different stages of plant growth and in vitro antibacterial activity of Thymus vulgaris Lamiaceae, from Iran. Beni-Suef University Journal of Basic and Applied Sciences. 3 (2): 87-92. Doi: https://doi.org/10.1016/j.bjbas.2014.05.001.
Osama D, El-Mahallawy H, Mansour MT, Hashem A, Attia AS. (2021). Molecular characterization of carbapenemase producing klebsiella pneumoniaee isolated from Egyptian pediatric cancer patients including a strain with a rare gene-combination of βlactamases. Infection and Drug Resistance. 14: 335–348. Doi: https://doi.org/10.2147/IDR.S284455
Podschun R and Ullmann U. (1998). Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors. Clinical Microbiology Reviews. 11 (4): 589-603. Doi: https://doi.org/10.1128/cmr.11.4.589
Poirel L, Walsh TR, Cuvillier V, Nordmann P. (2011). Multiplex PCR for detection of acquired carbapenemase genes. Diagnostic Microbiology and Infectious Disease. 70 (1): 119-123. Doi: https://doi.org/10.1016/j.diagmicrobio.2010.12.002
Shahid M, Ahmad N, Saeed NK, Shadab M, Joji RM, Al-Mahmeed A, Bindayna KM, Tabbara KS, Dar FK. (2022). Clinical carbapenem-resistant Klebsiella pneumoniae isolates simultaneously harboring bla NDM-1, bla OXA types and qnrS genes from the Kingdom of Bahrain: resistance profile and genetic environment. Frontiers in Cellular and Infection Microbiology. 12: 1033305. Doi: https://doi.org/10.3389/fcimb.2022.1033305.
Lobo AS and Moosabba M. (2020). Antibiogram and hypermuco viscosity pattern among Klebsiella pneumoniae isolate from respiratory samples: A tertiary care hospital study in South India. IP International Journal of Comprehensive and Advanced Pharmacology. 4 (4): 134-138. https://doi.org/10.18231/j.ijcaap.2019.028.
Yamamoto S, Terai A, Yuri K, Kurazono H, Takeda Y, Yoshida O. (1995). Detection of neurovirulence factors in Escherichia coli by multiplex polymerase chain reaction. FEMS Immunology and Medical Microbiology, 12 (2): 85-90. Doi: https://doi.org/10.1016/0928-8244(95)00053-A.
Plant, Algae, and Environment
Volume 7, Issue 2
2023
Pages 1127-1138

Files

Share

How to cite

Statistics