Evaluation of the Hormonal Changes in the Model Plant Arabidopsis thaliana as the Consequence of Pseudomonas aeruginosa Infection

Document Type : Original Article

Authors

1 Department of Cellular and Molecular Biology Faculty of Life Sciences and Biotechnology Shahid Beheshti University Postal Code: 1983969411

2 Department of Cellular and Molecular Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran

Abstract

Plants sense the microbial pathogen by perceiving the microbial signals through their highly specific immune receptors. These immune receptors are located on the plasma membrane and sense all types of microbes. Research over the last twenty years has shown the importance of Arabidopsis thaliana for the study of microbe-host interactions. The ability to sequence whole genomes of Arabidopsis thaliana has changed our view of biology. Here, the study of hormonal changes under the influence of biological stress of Pseudomonas aeruginosa bacteria was investigated. The bacterium P. aeruginosa was isolated from oil, cultured in a laboratory environment, and then sprayed onto Arabidopsis plants for stress evaluation. 24 hours after the application of stress, the hormones were measured by high-performance liquid chromatography (HPLC). According to the results, all hormones involved in the plant's immune system were significantly altered in response to stress by P. aeruginosa. The study revealed that the hormons salicylic, gibberellic acid, and jasmonic acid were the most altered compared to the control plants. While high levels of the hormones salicylic acid, gibberellic acid, and jasmonic acid hormones were observed, in other hormones such as melatonin and abscisic acid did not show asignificant changes. Among the elevated hormones, the levels of the hormones salicylic acid and jasmonic acid were highly statistically significant compared to the controls. These results confirm the specificity of hormone activation and P. aeruginosa specifically activates only defense-related hormones. Moreover, the findings of this study can be used for subsequent insvetigations on microbe-host interactions and future microbe infection control programs.

Keywords

References

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Plant, Algae, and Environment
Volume 7, Issue 2
2023
Pages 1213-1227

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