Crocus (Iridaceae) Species Relationships Based on the Anatomical Characters

Document Type : Original Article

Authors

1 Faculty of Life Sciences & Biotechnology, Shahid Beheshti University, Tehran, Iran

2 Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran e-mail: f_koohdar@yahoo.com

3 Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid, Beheshti University, Tehran, Iran.

4 Professor, Faculty of Biological Sciences and Biotechnology, Shahid Beheshti University

5 Department of Plant Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.

10.48308/jpr.2024.236429.1084

Abstract

Crocus belongs to the Iridaceae family which comprises 88-100 species. This genus is represented in Iran by 24 species. This study analyzed relationships among the eight taxa distributed in Iran using 11 leaf anatomical characters. UPGMA (Unweighted Paired Group using average) and WARD clustering were performed using the Euclidean distance after 1000 times bootstrapping. The principal component analysis (PCA) shows that the three main components represent 85% of the variation. The PCA of anatomical traits effectively delineated the species under investigation. Furthermore, the PCA Biplot demonstrated that anatomical features can successfully differentiate between species.  Notably, the condition of C. haussknechtii was found to be closely related to the two species, C. biflorus and C. michelsonii, despite C. haussknechtii being classified within the Crocus section, while the other two species belong to the Nudicapus section. Our findings indicate that additional anatomical data and molecular data are necessary for reliable conclusions regarding the studied species.

Keywords

References

Alavi-Kia SS, Mohammadi SA, Aharizad S, Moghaddam M. (2008). Analysis of Genetic Diversity and Phylogenetic Relationships in Crocus Genus of Iran Using Inter-Retrotransposon Amplified Polymorphism. Biotechnol & Biotechnol Eq 22: 795-800.
Anabat MM, Sheidai M, Riahi H, Koohdar F. (2022). A new look at the genus Crocus L. phylogeny and speciation: Insight from molecular data and chromosome geography. Genetic Resources and Crop Evolution. 69 (2): 855–870.
Butnariu, M., Quispe, C., Herrera-Bravo, J., Sharifi-Rad, J., Singh, L., Aborehab, N. M., Bouyahya, A., Venditti, A., Sen, S., & Acharya, K. (2022). The pharmacological activities of Crocus sativus L.: A review based on the mechanisms and therapeutic opportunities of its phytoconstituents. Oxidative Medicine and Cellular Longevity, 2022 (1), 8214821.
Caiola M G, Caputo P, Zanier R (2004). RAPD analysis in Crocus sativus L. accessions and related Crocus species. Biologia Plantarum 48: 375-380.
Coskun F, Selvi S, Satil F (2010). Phylogenetic relationships of some Turkish Crocus (Iridaceae) taxa based on morphological and anatomical characters. Turk J Bot 34: 1710-178.
Dolatyari, A., Tolyat Abolhasani, M., Ardalani, F., & Rukšāns, J. (2024). A taxonomic revision of the genus Crocus (Iridaceae) in Iran. Nordic Journal of Botany, e04270.
Gebrehiwot, K., Woldu, Z., Fekadu, M., Teferi, E., Desalegn, T., & Demissew, S. (2020). Classification and ordination of plant communities in Abune Yosef mountain range, Ethiopia. Acta Ecologica Sinica, 40(5), 398–411.
Golshani, F., Fakheri, B. A., Solouki, M., Mahdinezhad, N., & Feriz, M. R. K. (2020). Evolutionary and phylogenic relationships of wild and crop species of iranian saffron by DNA barcoding. Bangladesh Journal of Botany, 49(2), 287–296.
Hamer Øm., Harper DAT, Ryan P D (2012). PAST: Paleontological Statistics software package for education and data analysis. Palaeontologia Electronica 4: 9.
Harpke D, Meng S, Kerndorff H, Rutten T, Blattner FR (2013). Phylogeny of Crocus (iridaceae) based on one chloroplast and two nuclear loci: ancient hybridization and chromosome number evolution. Mol Phylogenetics Evol 66: 617-627.
Hammer, Ø., & Harper, D. A. (2024). Paleontological data analysis. John Wiley & Sons.
Jensen WA (1962). Botanical histochemistry: principles and practice. W. H. Freeman and Co, San Francisco.
Kerndorff H, Pasche E(1996b). Crocus from Turkey to Jordan (Part2). Quart Bull Alp Gard Soc 64: 459-467.
Kerndorff H, Pasche E (1997). Two remarkable taxa of the Crocus biflorus complex (Iridaceae) from northeastern Turkey. Linzer BiolBeitr 29: 591-600.
Kamra K, Jung J, Kim JH. (2023). A phylogenomic study of Iridaceae Juss. Based on complete plastid genome sequences. Frontiers in Plant Science. 14: 1066708.
Munirah MP, Norhayati, MN, Noraini M. (2022). Crocus sativus for insomnia: A systematic review and meta-analysis. International Journal of Environmental Research and Public Health. 19 (18): 11658.
Nemati Z, Blattner F R, Kerndorff H, Erol O, Harpke D (2018). Phylogeny of the saffron-crocus species group, Crocus series Crocus (Iridaceae). Mol Phylogenet. Evol. 127: 891-897.
Ljubisavljević, I., & Raca, I. (2020). Comparative morphological and leaf anatomical analysis of the species Crocus danubensis and Crocus variegatus (Iridaceae). Biologia, 75(3), 381–391.
Özen F, Temeltaş H, Aksoy Ö (2012). The Anatomy and Morphology of the Medicinal Plant Lilium candidum L. (Liliaceae), Distributed in Marmara Region of Turkey. Pak J Bot 44(4): 1185-1192.
Pasche E (1994a). A new Crocus (Iridaceae) from Turkey. Herbertia 49:67-75.
Petersen G, Seberg O, Thorsøe S, Jørgensen T, Mathew B (2008). A phylogeny of the genus      Crocus (Iridaceae) based on sequence data from five plastid regions. Taxon. 57 (2): 487-499.
Pedro de Padua, G. A., Chaves, L, Braga, G. G., Carmona, F., & Pereira, A. M. S. (2023). Effect of Crocus sativus L.(saffron) and crocin in the treatment of patients with type-2 diabetes mellitus: A systematic review and meta-analysis. Journal of Ethnopharmacology, 117255.
Podani J (2000). Introduction to the exploration of multivariate data. Backhuys Publishers, Leiden, Netherlands, 470 pp.
Pegg, T. J., Gladish, D. K., & Baker, R. L. (2021). Algae to angiosperms: Autofluorescence for rapid visualization of plant anatomy among diverse taxa. Applications in Plant Sciences, 9(6), e11437.
Raycheva, T., Stoyanov, K., Naimov, S., & Apostolova-Kuzova, E. (2021). Crocus adamioides (Iridaceae) in the Bulgarian flora. Acta Botanica Croatica, 80(1), 82–90.
Rudall P, Mathew B (1990). Leaf anatomy in Crocus (Iridaceae). Kew Bull 45(3): 535-544.
Rudall P (1994) Anatomy and systematics of Iridaceae. Bot J Linn. Soc 114: 1-21.
Seberg O, Petersen G. 2009. How many loci does it take to barcode a crocus? PLoS ONE 4, e4598.
Raycheva, T., Stoyanov, K., Naimov, S., & Apostolova-Kuzova, E. (2023). Crocus heuffelianus—A New Species for the Bulgarian Flora from Series Verni (Iridaceae). Plants, 12(13), 2420.
Siracusa, L., Onofri, A., Galesi, R., Impelluso, C., Pulvirenti, L., Ruberto, G., Gresta, F., Spampinato, G., & Cristaudo, A. (2022). An integrated approach for the characterization of wild Crocus species adopting phenotypical and phytochemical traits. Phytochemistry, 202, 113315.
 
Torres-Pio, K., De la Cruz-Guzmán, G. H., Arévalo-Galarza, M. de L., Aguilar-Rodríguez, S., Grego-Valencia, D., Arriaga-Frías, A., & Mandujano-Piña, M. (2021). Morphological and anatomical changes in Lilium cv. Arcachon in response to plant growth regulators. Horticulture, Environment, and Biotechnology, 62, 325–335.
Uslu, E., Babaç, M. T., & Bakış, Y. (2022). Investigations on anatomical and morphological characteristics of some Crocus L. taxa around Abant Lake. Anatolian Journal of Botany, 6(2), 122–131.
Yılmaz, A. (2021). The evaluations and comparisons of nuclear and chloroplast DNA regions based on species identification and phylogenetic relationships of Crocus L. taxa. Journal of the Institute of Science and Technology, 11(2), 1504–1518.
Yazici, C., Çiftçi, A., Mollman, R., Harpke, D., & Erol, O. (2024). Diversity of Crocus gargaricus sl: Resolving longstanding debates and discovering a new species. Plant Systematics and Evolution, 310(4), 28.
Zdemir AYC, Zdemir AC, Zdemir CC (2010). Statistical Comparative Leaf Anatomy of Some Crocus L. Taxa. Asian J. Math. 3 (l): 16-24.
Plant, Algae, and Environment
Volume 8, Issue 2
DOi
September 2024
Pages 1347-1355

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