The Efficacy of Different Concentrations of Gibberellic Acid on Growth Rate, Dry Biomass, Endogenous Gibberellic Acid, Lipid Content, and Some Photosynthetic Pigments of Nannochloropsis salina Microalgae in Guillard (f/2) Medium

Document Type : Original Article

Authors

1 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Zabol University, Zabol, Iran Department of Biotechnology,The persian Gulf University,Bushehr.Iran

2 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Zabol University, Zabol, Iran

3 Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural

4 Department of Plant Protection, Faculty of Agriculture, Zabol University, Zabol, Iran

5 Department of Plant Genetics and Breeding, Faculty of Agriculture and Natural Resources, Persian Gulf University, Bushehr, Iran

Abstract

Nannochloropsis salina is a promising candidate for lipid and biomass production used in aquaculture and biofuel production. On the other hand, phytohormones have different effects on the metabolism of single-celled microalgae species. One of the objectives of this study was to investigate the impact of different gibberellic acid (GA) treatments on growth, some photosynthetic pigments, dry biomass, endogenous GA level, and total lipid content in N.salina microalgae in a Guillard (F/2) medium. The experimental treatments included 0 (control), 0.5, 5, 10, and 20 mg L-1 of GA concentrations. This experiment was conducted in a completely randomized design (CRD) with three replications during 2021-2022 in the Persian Gulf Microalgae Development Laboratory at Persian Gulf Science and Technology Park, Bushehr, Iran. The measured traits included growth rate, doubling time, chlorophyll a and b content, total carotenoid, dry biomass, endogenous GA level, and total lipid content. The analysis of variance (ANOVA) showed that GA treatments had a significant effect on all studied traits at the level of (p<0.01). The exogenous application of GA treatment increased the growth rate and the rate doubled to 0.085 per day and 8.2 days respectively, with the content of chlorophyll b with an average of 2.07 µg/g DW, total chlorophyll to the amount of 72 4.0 µg/g DW in N. salina microalgae, and the highest amount of total lipid and dry biomass with 24.69% and 0.79 g/L were observed in 20 mg/L treatment, respectively. While the highest growth rate was obtained at the concentration of 0.5 mg/L GA. It is suggested that a concentration of 20 mg/L GA be used in the exponential growth phase to increase biomass production and total lipid content in N. salina under Guillard (f/2) medium. 

Keywords

References

Aftab T, Khan M, Idrees M, Naeem M. (2011). Optimizing nitrogen levels combined with GA for enhanced yield, photosynthetic attributes, enzyme activities, and artemisinin content of Artemisia annua. Frontiers of Agriculture in China. 5(1): 51-59. DOI:10.1007/s11703-011-1065-7.
Chia MA, Lombardi AT, MELãO MD. (2013). Growth and biochemical composition of Chlorella vulris in different growth media. Anais da Academia Brasileira de Ciências. 85(04): 1427-1438. DOI:10.1590/0001-3765201393312
Du H, Ahmed F, Lin B, Li Z, Huang Y, Sun G, Ding H, Wang C, Meng C, Gao Z. (2017). The effects of plant growth regulators on cell growth, protein, carotenoid, PUFAs and lipid production of Chlorella pyrenoidosa ZF strain. Energies. 10 (11): 1696. DOI:10.3390/en10111696
Falkowska M, Pietryczuk A, Piotrowska A, Bajguz A, Grygoruk A, Czerpak R. (2011). The effect of gibberellic acid (GA3) on growth, metal biosorption and metabolism of the green algae Chlorella vulgaris (Chlorophyceae) Beijerinck exposed to cadmium and lead stress. Polish journal of Environmental Studies. 20 (1):53-9.
Gao Z, Meng C, Zhang X, Xu D, Miao X, Wang Y, Yang L, Lv H, Chen L, Ye N. (2012). Induction of salicylic acid (SA) on transcriptional expression of eight carotenoid genes and astaxanthin accumulation in Haematococcus pluvialis. Enzyme and Microbial Technology. 51(4):225-30. DOI:10.1016/j.enzmictec.2012.07.001
Ghasemi M, Modarresi M, Babaeian Jelodar N, Bagheri N, Jamali A. (2016). The evaluation of exogenous application of salicylic acid on physiological characteristics, proline and essential oil content of chamomile (Matricaria chamomilla L.) under normal and heat stress conditions. Agriculture. 6(3):31. DOI:10.3390/agriculture6030031
Lichtenthaler HK. (1988). In vivo chlorophyll fluorescence as a tool for stress detection in plants. In Applications of chlorophyll fluorescence in photosynthesis research, stress physiology, hydrobiology and remote sensing (pp. 129-142). Springer. DOI:10.1007/978-94-009-2823-7_16
Lin B, Ahmed F, Du H, Li Z, Yan Y, Huang Y, Cui M, Yin Y, Li B, Wang M, Meng C. (2018). Plant growth regulators promote lipid and carotenoid accumulation in Chlorella vulgaris. Journal of Applied Phycology. 30:1549-61. DOI:10.1007/s10811-017-1350-9
Liu S, Chen W, Qu L, Gai Y, Jiang X. (2013). Simultaneous determination of 24 or more acidic and alkaline phytohormones in femtomole quantities of plant tissues by high-performance liquid chromatography–electrospray ionization–ion trap mass spectrometry. Analytical and bioanalytical chemistry. 405:1257-66. DOI:10.1007/s00216-012-6509-2
Madani NS, Shamsaie Mehrgan M, Hosseini Shekarabi SP, Pourang N. (2021). Regulatory effect of gibberellic acid (GA3) on the biomass productivity and some metabolites of a marine microalga, Isochrysis galbana. Journal of Applied Phycology. 33(1):255-62. DOI:10.1007/s10811-020-02291-1
Mansouri H, Asrar Z, Amarowicz R. (2011). The response of terpenoids to exogenous GA in Cannabis sativa L. at vegetative stage. Acta Physiologiae Plantarum. 33(4): 1085-1091. DOI:10.1007/s11738-010-0636-1.
Mansouri H, Talebizadeh, B. (2016). Effect of GA on the cyanobacterium Nostoc linckia. Journal of Applied Phycology. 28 (4): 2187-2193. DOI:10.1007/s10811-015-0756-5.
Moradi F, Sarmad J, Ghafoori H, Naeemi AS. (2015). Total lipids content in the green microalgae of Chlorella sp. under nitrogen starvation stress. Aquatic Physiology and Biotechnology. 3 (1):1-3.
Osório C, Machado S, Peixoto J, Bessada S, Pimentel FB, C. Alves R, Oliveira MB. (2020). Pigments content (chlorophylls, fucoxanthin and phycobiliproteins) of different commercial dried algae. Separations. 7 (2): 33. DOI:10.3390/separations7020033.
Pan X, Chang F, Kang L, Liu Y, Li G, Li D. (2008). Effects of gibberellin A3 on growth and microcystin production in Microcystis aeruginosa (cyanophyta). Journal of Plant Physiology. 165(16): 1691-1697. DOI:10.1016/j.jplph.2007.08.012.
Park WK, Yoo G, Moon M, Kim CW, Choi YE, Yang JW. (2013). Phytohormone supplementation significantly increases the growth of Chlamydomonas reinhardtii cultivated for biodiesel production. Applied Biochemistry and Biotechnology. 171 (5): 1128-1142. DOI:10.1007/s12010-013-0386-9.
Salmaninejad M. (2016). Effect of culture mediums and light intensity on growth and carotenoids of Dunaliella salina in Urmia Lake. Journal of Plant Research (Iranian Journal of Biology). 28 (4): 771-783.
Sivaramakrishnan R and Incharoensakdi A. (2020). Plant hormone-induced enrichment of Chlorella sp. ome-3 fatty acids. Biotechnology for Biofuels. 13 (1): 1-14. DOI:10.1186/s13068-019-1647-9.
Stirk W, Tarkowská D, Gruz J, Strnad M, Ördög V, van Staden J. (2019). Effect of gibberellins on growth and biochemical constituents in Chlorella minutissima (Trebouxiophyceae). South African Journal of Botany. 126: 92-98. DOI:10.1016/j.sajb.2019.05.001.
Trinh CT, Tran TH, Bui TV. (2017). Effects of plant growth regulators on the growth and lipid accumulation of Nannochloropsis oculata (droop) Hibberd. AIP Conference Proceedings. 1878: 1. DOI: 10.1063/1.5000185.
Do TC, Tran DT, Le TG, Nguyen QT. (2020). Characterization of endogenous auxins and gibberellins produced by Chlorella sorokiniana TH01 under phototrophic and mixtrophic cultivation modes toward applications in microalgal biorefinery and crop research. Journal of Chemistry. 2020 (1):4910621. DOI: 10.1155/2020/4910621.
Verma I, Roopendra K, Sharma A, Chandra A, Kamal A. (2019). Expression analysis of genes associated with sucrose accumulation and its effect on source-sink relationship in high sucrose accumulating early maturing surcane variety. Physiology and Molecular Biology of Plants. 25 (1): 207-220. DOI: 10.1007/s12298-018-0627-z.
Wen Y, Su Sc, Ma Ly, Wang Xn. (2018). Effects of GA on photosynthesis and endogenous hormones of Camellia oleifera Abel. in 1st and 6th leaves. Journal of Forest Research. 23 (5): 309-317. DOI: 10.1080/13416979.2018.1512394.
Yu XJ, Sun J, Sun YQ, Zheng JY, Wang Z. (2016). Metabolomics analysis of phytohormone gibberellin improving lipid and DHA accumulation in Aurantiochytrium sp. Biochemical Engineering Journal. 112: 258-268. DOI:10.1016/j.bej.2016.05.002.
Zarandi-Miandoab L, Hejazi MA, Bagherieh-Najjar MB, Chaparzadeh N. (2015). Light intensity effects on some molecular and biochemical characteristics of Dunaliella salina. Physiology. 5 (2):1311-21.
Plant, Algae, and Environment
Volume 8, Issue 2
2024
Pages 1477-1492

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