Plant, Algae, and Environment

Plant, Algae, and Environment

A Biosystematics Study of Microcystis (Cyanobacteria), A Bloom-Forming Cyanobacterium from Aras Reservoir (North-West Iran)

Authors
Fereydon Mohebbi 1
Ahmad Ghoroghi 1
ُsohrab RezvaniGilkolaei 2
Hossein Riahi 3
Ali Nekoeifard 2
Masoud Seidgar 1
1 Assistant Prof., National Artemia research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Uramia, Iran
2 Associate Prof., National Artemia research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Uramia, Iran
3 Faculty of Biological Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
Abstract
Today, harmful cyanobacerial bloom is consideredas a main problem in freshwater ecosystemsall over the world. The Microcystis is an importantgenus in cyanobacteria composed of about 23species. This genusforms various shaped coloniesincluding spherical or oblong cells which wholecolony is surrounded by a mucilage sheath. Manypopulations and species of the genus produce severblooms and often toxins in eutrophic freshwaters.The genus Microcystis has been defined based onmorphological, morphometrical and genetical criteria,however, its classification under the genuslevel is still indistinct and presence of traditionalmorphological species within the genus is doubtful.Therefore, determining of the natural diversityunder the genus level in Microcystis has a crucialimportance. The purpose of this study was to determinethe species of Microcystis in Aras reservoir.The samples were collected from 6 sampling siteson 18 August 2013. Morphological and morphometricalstudies of Microcystis was carried out byNikon 50i microscope equipped with Nikon DSFicamera and Nikon monitor with length measurmentsoftware. Principal Component Analysis(PCA) and two-way Unweighted Pair GroupMethod with Arithmetic Mean (UPGMA) byPAleontological STatistics (PAST) version 3.04were performed to determine biosystematics variationsbetween Microcystis populations. The resultsindicated that Microcystis from Aras reservoirwas composed of two distinct groups based on theshape and size of cells, colony form, presence andabsence of gelatinous sheath around the colonies,etc).These two groups may be considered as twomorphospecies of M. botrys which are separateddue to different ecological factors.
Keywords
Microcystis
Morphology
Morphometry
Aras reservoir
  1. Bittencourt-Oliveira MC. (2000). Development of
  2. Microcystis aeruginosa Kütz. (Cyanophyceae/ Cyanoacteria)
  3. under cultivation and its taxonomic implications.
  4. Archives for Hydrobiology/Algology Studies. 99: 29-37.
  5. Chorus I and Bartram J. (1999). Toxic cyanobacteria
  6. in water: a guide to their public health consequences,
  7. monitoring and management. E & F Spon,
  8. London.
  9. Crow WB. (1923). The taxonomy and variation of the
  10. genus Microcystis in Ceylon. New Phycologist. 22:59- 68.
  11. Desikachary IV. (1959). Cyanophyta. I.C.A.R., New
  12. Delhi. 685 pp.
  13. Doolittle WF. (1982). Molecular evolution. In: The
  14. Biology of cyanobacteria, Volume 19. N. G. Carr and
  15. B. A. Whitton, eds. Berkeley, University of California
  16. Press. p. 307-332.
  17. Filipuzzi S and Faramarzi M. (2007). The Science and
  18. Politics of Large Dam Project Aras river basin: dam
  19. facility and water management policy. An analysis on
  20. performance. Swiss Federal Institute of Aquatic Scenceand
  21. Technology.
  22. Geitler L. (1932). Cyanophyceae. In Kryptogramen
  23. flora von Deutschland, Österreich und der Schweiz.
  24. Vol. 14, ed. by Rabenhorst L., Akademisce Verlags
  25. gesell schaft, Leipzig. pp. 130–148.
  26. Gibbons NE and Murray RGE. (1978). Proposals concerning
  27. the higher taxa of bacteria. International
  28. Journal of Systematic Bacterioliology. 28: 1-6.
  29. Hammer Ø, Harper DAT and Ryan PD. (2001). PAST:
  30. Paleontological Statistics software package.
  31. Holt JG, Krieg NR, Sneath PHA, Staley JT,
  32. Williams ST. (1994). Group 11.Oxygenic phototrophic
  33. bacteria. In Bergey’s Manual of Determinative
  34. Bacteriology, 9th ed., edited by Hensyl, W. R., Wil
  35. liams & Wilkins, Baltimore, pp. 377–425.
  36. Komarek J and Komarkova J. (2002). Review of the European
  37. Microcystis morphospecies (Cyanoprokary
  38. otes) from nature. Czech Phycology. Olomouc. 2: 1-
  39. Otsuka S, Suda S, Li R, Matsumoto S, Watanabe MM.
  40. (2000). Morphological variability of colonies of
  41. Microcystis morphospecies in culture. Journal of General
  42. and Applied Microbiology. 46:39-50.
  43. Reynolds CS, Jaworski GHM, Cmiech HA, Leedale
  44. GF. (1981). On the annual Cycle of the blue-green algae
  45. Microcystis aeruginosa Kütz. Emend. Elenkin. Philosophy
  46. of Transaction of the Royal Society London
  47. B. 293:419-477.
  48. Schopf JW. (2000). The fossil record: tracing the roots
  49. of the cyanobacterial lineage. In: Whitton BA, Potts M
  50. (Eds) The ecology of cyanobacteria. Kluwer Academic,
  51. Dordrecht. Pp. 13–35.
  52. Somdee Th, Kaewkhiaw K, Somdee A. (2013). Detection
  53. of toxic cyanobacteria and quantification of microcystins
  54. in four recreational water reservoirs in Khon
  55. Kaen, Thailand. KKU Research Journal. 18: 1-8.
  56. Stanier RY, Kunisawa R, Mandel M, Cohen-Bazire G.
  57. (1971). Purification and properties of unicellular bluegreen
  58. algae (order Chroococcales). Bacteriological Reviews.
  59. : 171–205.
  60. Stanier RY, Sistrom WR, Hansen TA, Whitton BA, Castenholz
  61. RW, Pfenning N, Gorlenko VN, Kondratieva
  62. EN, Eimhjellen KE, Whitten Bury R, Gherna RL, Truper
  63. HG. (1978). Proposal to place
  64. the nomenclature of the cyanobacteria (blue-green algae)
  65. under the rules of the International Code of Nomenclature
  66. of Bacteria. International Journal of Systematic
  67. Bacteriology. 28: 335-336.
  68. Tahami FS. (2001). Toxic characteristics of Microcystis.
  69. South physician, vol. 4, special issue of national physician
  70. and sea congress. Pp.60.