Screening of Thermophilic Cyanobacteria from some hot Springs of Iran

Authors

1 Faculty of life sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran

2 Faculty of life sciences and biotechnology, ShahidbBeheshti University, Tehran, Iran

3 Faculty of life sciences and biotechnology, Shahid Beheshti University, Tehran, Iran

4 Faculty of Basic Science, Hormozgan University, Bandar Abbas, Iran

Abstract

Hot springs have been a subject of intensediscussion for biologists in the last decades.The polyphasic approach is the most progressivesystem that has been suggested for distinguishingand phylogenetically classifying cyanobacteria.In a revision of the cyanobacteria inhot springs of Iran, four hot springs of Iran wereinvestigated. We developed a combined molecularand morphological approach to identificationof cyanobacteria. In this study seventeenpopulations and 13 morphological characterswere analyzed. Molecular study based on 16SrRNA gene sequence does not disrupt morphologicalinformation and it confirms the separationof studied taxa according to morphologicalcharacters. Based on their growth characteristics;seven species were selected for the productionof antimicrobial agents. The results ofthis study showed that the methanol extractsexhibited high antimicrobial activity againstsome gram positive bacteria, moderate antibacterialactivity against some gram negativeorganisms and moderate antifungal activityagainst some fungi. Four species were selectedfor salinity tolerance. The results showed hightolerance of these taxa to salinity.

Keywords

References

  1. References
  2. Bock TD. (1978). Thermophilic microorganisms and life at high temperatures. Berlin, Springer Verlag.
  3. Castenholz RW. (1996). Endemism and biodiversity of thermophilic cyanobacteria. Nova Hedwigia, Beiheft. 112: 33-47.
  4. Castenholz RW. (2001). Phylum BX. Bacteria- In: Boone, D.R. and Castenholz, R.W. (eds): Bergey s Manual of Systematic Bacteriology, 2 ndEdition, Springer Verlag. 473-599.
  5. Ferris M, Ruff-Roberts A, Kopczynski E, Bateson M, Ward D. (1996). Enrichment culture and microscopy ceneceal diverse thermophilic Synechococcus populations in a single hot spring microbial mat habitat. Applied Environmental Microbiology. 62: 1045-1050.
  6. Ghozzi K, Zemzem M, Dhiab R, Challouf R, Yahia A, Omrane H, Ben Ouada H. (2013). Screening of thermophilic microalgae and cyanobacteria from Tunisian geothermal sources. Journal of Arid Environments. 97: 14-17.
  7. Giovannoni SJ, Turner S, Olsen GJ, Barns S, Lane DJ, Pace NR. (1988). Evolutionary relationships among cyanobacteria and green chloroplasts. Bacteriology. 170 (8): 3584-3592.
  8. Hongmei J, Aitchison J. C, Lacap D. C, Peerapornpisal Y, Sompong U, Pointing S. B, (2005). Community phylogenetic analysis of moderately thermophilic cyanobacterial mats from China, the Philippines and Thailand. Extremophiles. 9: 325–32.
  9. Jonathan F and John F.S, 2009. Flat laminated microbial mat communities. Earth-Science Reviews 96, 163-172.
  10. Komarek J and Anagnostidis K. (1989). Modern approach to the classification system of cyanophytes, 4-Nostocales. Archive for Hydrobiology, Algology studies. Suppl. 82, 56: 247-345.
  11. Komarek J and Anagnostidis K. (1998). Cyanoprokaryota. 1. Teil: Chroococcales.In: Ettl, H.; Gardner, G.; Heying, H. and Mollenhauer, D (eds.): Susswasserflora von Mitteleuropa, Gustav Fischer, Jena- Stuttgart- Lubeck- Ulm. 19(1): 548 pp.
  12. Komarek J and Anagnostidis K. (2005). Cyanoprokaryota.2 Teil: Oscillatoriales,- In: Budel B., Gardner G., Krienitz l. and Schagerl M. (eds): Susswasserflora von Mitteleuropa. 19 (2): 759 pp.
  13. Marteinsson VT, Hauksdottir S, Hoble CFV, Kristamannsdottri H, Hreggvidsson GO, Kristiansson JK. (2001). Phylogenetic diversity analysis of subterranean hot springs in iceland. Applied. Environmental Microbiology. 67: 4242–8.
  14. National Committee for Clinical Laboratory Standard (NCCLS). (1999) Performance standards for Scientific antimicrobial susceptibility testing. 9th International Supplement. M100-S9.Wayne, Pensylvania, USA.
  15. Norris TB, Mcdermott TR, Castenholz RW. (2002). The long-term effects of UV exclusion on the microbial composition and photosysthetic competence of cyanobacteria in hot spring microbial mats. FEMS Microbiology Ecology. 1323: 1–17.
  16. Skimisdottir S, Hreggvidsson GO, Hiorleifsdottir S, Marteinsson VT, Petursdottir S.K, Holst O, Kristjansson JK. (2000). Influence of sulfide and temperature on species composition and community structure of hot spring microbial mats. Applied Environmental Microbiology. 66: 2835-2841.
  17. Soltani N, Zarrini G, Ghasemi Y, ShokraviSh, Baftehchi L. (2007). Characterization of a soil cyanobacterium Fischerella sp. FS 18 under NaCl stress. Journal of Biological Sciences 7 (6):931-936.
  18. Sompong U, Anuntalabhochai S, Cutler R. W, Castenholz R. W, Peerapornpisal Y. (2008). Morphological and Phylogenic diversity of cyanobacterial populations in six hot springs of Thailand. Science Asia. 34: 153-162.
  19. Sompong U, Hawkins P, Besley C, Peerapompisal Y. (2005). The distribution of cyanobacteria across Physical and chemical gradients in hot spring in northern Thailand. FEMS Microbiology Ecology. 52: 365-376.
  20. Stanier RY, Kunisawa R, Mandel M, Cohen- Bazire G. (1971). Purification and properties of unicellular blue- green algae (order Chroococcales). Bacteriology Review. 35: 171-205.
  21. Stoyanov P, Moten D, Mladenov R, Dzhambazov B, Teneva I. (2014). Phylogenetic Relationships of Some Filamentous Cyanoprokaryotic Species. Evolutionary Bioinformatics. 10: 39–49.
  22. Ward DM and Castenholz RW. (2000).Cyanobacteria in geothermal habitats in Ecology of cyanobacteria. Their diversity in time and space (Whitton B.A. and Potts M., eds.), -Dordrecht, Kluwer Academic Publishers. 37-59.
  23. Zakaria AM and Abdulrahman MA. (2007). Cyanobacteria and their toxins in treatedwater storage reservoirs in Abha city, Saudi Arabia. Elsevier. Toxicon. 50: 75-84.

Files

Share

How to cite

Statistics