Impact of Salinity and pH on Several Species of Anabaena (Nostocaceae, Nostocales) Isolated from Rice Fields in Iran

Authors

1 Faculty of Life Sciences and Biotechnology, University of Shahid Beheshti, Evin, Tehran, Iran

2 Faculty of Life Sciences and Biotechnology, University of Shahid Beheshti, Evin, Tehran, Iran.

Abstract

The purpose of this study is to developa biofertilizer based on filamentous nitrogen-fixing cyanobacteria selected from ricefields and to generate a technological packagecompatible with its use for the rice cropin Iran. Cyanobacteria was isolated and purifiedfrom rice fields in Kalate Naderi. Inthis research we studied the effect of salinity(NaCl, 0, 1, 2 and 4%) and pH (5, 7, 9 and11) on growth and chlorophyll-a contentsin six species of Anabaena. Results showedthat Anabaena sphaerica Bornet & Flanaultpossessed the best adaptation to pH changes.It could be more active in 5-11 pH values.A. vaginicola F.E. Fritsh & Rich andA. variabilis Kutzing ex Bornet & Flanaultwere remarkable for salinity tolerance. Theyadapted to salinity stress up to 2% salt concentrationin the medium. Our results indicatedthat the growth of all strains decreaseby 4% salt concentration and pH 11. Indeed,Anabaena is a cyanobacterium with nitrogenfixation ability and high potency of adaptationto environmental stress. So, it canbe a useful candidate for biofertilizer in agriculture,particularly in rice fields.

Keywords

References

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